Pour Sample into Strainer
| SCSR Cell Isolation - Photographs |
| Stomacher Bag Filtration |
Pour Sample into Strainer
Extraction Region
Extract using Pipette
| 40µm Filtration |
Place filter over
50-ml centrifuge tube
To avoid clogging, repeatedly aspirate and dispense sample using pipette
| Underlay Cushion |
Slowly underlay
cushion medium from the bottom of tube. Try to avoid bubbles and
disturbance of the interface.
| Appearance after Centrifugation |
The tube after
centrifugation
| Remove Supernatant |
Use
pipette to remove supernatant, leaving 5-10 ml above interface
| Extracting the Interface |
Extract interface using plastic transfer pipette and dispense into a new tube. Slowly follow a
downward spiral path along tube wall while extracting.
If there is a 'tinge' to the top of the cushion, extract a small amount of
cushion as well.
Leave most of cushion with the pellet fraction. Fractions will be washed
in the next step.
Copyright©2005 NonInvasive Technologies, USA. All rights reserved